Imanis Life Sciences | CL176 | U266B1-Fluc-Puro/eGFP-Neo

品名：U266B1-Fluc-Puro/eGFP-Neo

货号：CL176

品牌：Imanis Life Sciences

产品描述

Species：Human

Cell Type：Multiple Myeloma

Transgene：Firefly luciferase (Fluc; Luc2);Emerald GFP (EmGFP)

Selection Gene：Puromycin;Neomycin

Parental U266B1 cells were transduced with LV-SFFV-Luc2-P2A-Puro (Imanis #LV012) and LV-SFFV-eGFP-P2A-Neo (Imanis #LV067), which encode the firefly luciferase (Fluc; Luc2) and enhanced green fluorescent protein (eGFP) cDNAs, respectively, under the spleen focus-forming virus (SFFV) promoter and linked to antibiotic resistance (puromycin, neomycin) genes via a P2A cleavage peptide. A high Fluc and eGFP expressing population was generated by selection using puromycin and G418 followed by selection with a methylcellulose based semi-solid medium.This cell line has been tested for Mycoplasma contamination and is certified mycoplasma free.The parental U266B1 cell line was licensed and purchased directly from ATCC*.*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.