Novocib S1200-03 | Yeast Extract Nucleotides Analysis

品名：Yeast Extract Nucleotides Analysis

货号：S1200-03

品牌：Novocib

产品描述

HPLC-UV analysis for full spectra of dietary nucleotides (bases, nucleosides and NMP, unhydrolyzed DNA and RNA nucleic acids) present in feed or ingredients.

Agilent 1120 series HPLC liquid chromatograph fitted with binary pump, vacuum degasser, well-plate autosampler, thermostatic column compartment and multiple wavelength and diode array detector. Run and data acquisision are controlled by Agilent Chem Station software. Calibrations are performed with standards prepared in mobile phase and with standards mixed with cell extracts, which are run immediately before and after every series of samples. Peak assignment of different bases, ribonucleosides and ribonucleoside monophosphatesis is done by comparing both retention times and characteristics of UV absorption spectra (254/280 ratio) with those of standards. The area of individual peaks was measured using ChemStation software (Agilent).