Novocib K0211-01 | Phosphatase Assay Kit 磷酸酶通用检测试剂盒

品名：Phosphatase Assay Kit

中文名称：磷酸酶通用检测试剂盒

货号：K0211-01

规格：1 plate (96 assays)

品牌：Novocib

产品描述

PRECICE® Phosphatase Assay Kit developed by NOVOCIB is a continuous spectrophotometric enzyme assay to monitor activity of any enzymes liberating inorganic phosphate (Pi) during enzymatic reactions. The kit can be used for:Phosphatase activity assay；Alkaline phosphatase activity；Protein phosphatases activity assay；ATPase assay；GTPases assay；Helicases assay.

The release of inorganic phosphate is measured through coupling to PNP-XDH enzymatic system. In the presence of inorganic phosphate, PNP (purine nucleoside phosphorylase) catalyzes inosine phosphorolysis leading to the formation of hypoxanthine and P-ribose. XDH enzyme catalyzes irreversible oxidation of hypoxanthine to uric acid with simultaneous reduction of NAD to NADH2, measurable by absorbance at 340nm. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm. The assay is linear in 5-300µM range.