Novocib E-Nov6 | Human cytosolic 5'-nucleotidase II (cN-II) 5'-核苷酸酶 II

品名：Human cytosolic 5'-nucleotidase II (cN-II)

中文名称：5'-核苷酸酶 II

货号：E-Nov6

规格：50 mUnits；150 mUnits

品牌：Novocib

产品描述

Synonyms: cytosolic 5'-nucleotidase/phosphotransferase, High Km 5'-nucleotidase (HKM-NT), cytosolic purine 5'-nucleotidase (purine 5'-NT), IMP/GMP-specific 5'-nucleotidase.

NOVOCIB'S human cytosolic IMP/GMP-specific 5'-nucleotidase/phosphotransferase II (cN-II) is a pure and active protein of 65kDa cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli. The sequence of the cloned NT5C2 gene (GenBank accession number P49902) was confirmed by DNA sequencing (100% identity).

Cytosolic 5'-nucleotidase II specifically catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates (IMP, dIMP, GMP, dGMP) and regulates cellular pools of IMP and GMP. The enzyme also acts as a phosphotransferase catalyzing phosphate transfer from nucleoside monophosphates to nucleoside acceptors, preferentially inosine and deoxyinosine. Unlike other 5'-nucleotidases, cN-II is allosterically regulated by adenine/guanine nucleotides and 2,3-biphosphoglycerate.

In addition, cN-II phosphorylates antiviral and antitumor nucleoside analogues such as 2'3'-dideoxyinosine, carbovir, acyclovir, and ribavirin.