| 产品名称 | ReVacc Scientific U90R79 | HIV-1 Trimeric GP140 protein-clade A, BG505 strain |
|---|---|
| 目录号 | U90R79 |
| 别名 | HIV-1 Trimeric GP140 protein-clade A, BG505 strain |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| U90R79 | 100 μg; 500 μg | 咨询 | 咨询 |
ReVacc Scientific U90R79 | HIV-1 Trimeric GP140 protein-clade A, BG505 strain
品名：HIV-1 Trimeric GP140 protein-clade A, BG505 strain
货号：U90R79
品牌：ReVacc Scientific
规格：100 μg；500 μg
产品描述
| Source | HEK293 cells | Tag | His |
| Organism | HIV-1 (virus caused AIDS) | Biosafety Level | BSL-1 |
| Applications | Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe | ||
| Storage | Recommend -80°C storage. Store at 4°C for short-term only. Multiple freeze/Thaw cycles will reduce its activity. | ||
A recombinant trimeric form of the HIV-1 glycoprotein (GP protein) from BG505 strain (clade A, GenBank: ABA61516.1) was produced by human embryonic kidney HEK293 cells, followed by Lectin purification. This product contains ectodomain of GP protein (end at D664) with furin cleavage site replaced by a linker, a T332N mutation for glycan, a I559P mutation for stable trimer formation, and C-terminal 8 hexa-histidine tag. This product has been evaluated by Size Exclusion Chromatography (SEC) to confirm the trimer formation, SDS-PAGE gel for purity and ELISAs for binding, see results. Due to the unique feature of BG505 at residue 241 glycan hole, a rabbit antibody 11B can target this protein (ref: Cell Rep. 2016;16(9):2327-38.), but not other HIV GP with 241N, such as YU2 (Catalog W44H24) or 16055 trimer (Catalog E7P43).
