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产品名称 ReVacc Scientific W44H24 | HIV-1 Trimeric GP140 protein-clade B, YU2 strain
目录号 W44H24
别名 HIV-1 Trimeric GP140 protein-clade B, YU2 strain
外观 见COA
分子量 N/A
CAS N/A
溶解度
存储条件 见COA
保存时间 见COA
备注1
备注2
目录号 规格 价格 库存状态  
W44H24 100 μg; 500 μg 咨询 咨询


ReVacc Scientific W44H24 | HIV-1 Trimeric GP140 protein-clade B, YU2 strain


品名:HIV-1 Trimeric GP140 protein-clade B, YU2 strain

货号:W44H24

品牌:ReVacc Scientific

规格:100 μg;500 μg


产品描述

SourceHEK293 cellsTagHis
OrganismHIV-1 (virus caused AIDS)Biosafety LevelBSL-1
ApplicationsCrystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others
StorageRecommend -80°C storage. Store at 4°C for short-term only.  Multiple freeze/Thaw cycles will reduce its activity.


A recombinant trimeric form of the HIV-1 glycoprotein (GP protein) from YU2 strain (clade B, GenBank: QHJ92204) was produced by human embryonic kidney HEK293 cells, followed by Lectin purification. This product contains ectodomain of GP protein with furin cleavage site knocked-down, T4 foldon trimerization domain, and C-terminal 6 hexa-histidine tag. Amino acid sequence is available upon request.  This product has been evaluated by Native Blue gel and Size Exclusion Chromatography (SEC) to confirm the trimer formation, SDS-PAGE for purity as well as ELISAs for binding, see results. A rabbit antibody 11B that targets 241 glycan hole (BG505 specific, catalog U90R79) does not to bind to this protein (ref: Cell Rep. 2016;16(9):2327-38.).

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