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产品名称 ReVacc Scientific E7P43 | HIV-1 Trimeric GP140 protein- clade C, 16055 strain
目录号 E7P43
别名 HIV-1 Trimeric GP140 protein- clade C, 16055 strain
外观 见COA
分子量 N/A
CAS N/A
溶解度
存储条件 见COA
保存时间 见COA
备注1
备注2
目录号 规格 价格 库存状态  
E7P43 100 μg; 500 μg 咨询 咨询


ReVacc Scientific E7P43 | HIV-1 Trimeric GP140 protein- clade C, 16055 strain


品名:HIV-1 Trimeric GP140 protein- clade C, 16055 strain

货号:E7P43

品牌:ReVacc Scientific

规格:100 μg;500 μg


产品描述

SourceHEK293 cellsTagHis
OrganismHIV-1 (virus caused AIDS)Biosafety LevelBSL-1
ApplicationsCrystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe
StorageRecommend -80°C storage. Store at 4°C for short-term only.  Multiple freeze/Thaw cycles will reduce its activity.


A recombinant trimeric form of the HIV-1 glycoprotein (GP protein) from 16055 strain (clade C, GenBank: ABL67444.1) was produced by human embryonic kidney HEK293 cells, followed by Lectin purification. This product contains ectodomain of GP protein (end at D664) with furin cleavage site replaced by a linker, a I559P mutation for stable trimer formation, and C-terminal 8 hexa-histidine tag. This product has been evaluated by Size Exclusion Chromatography (SEC) to confirm the trimer formation, SDS-PAGE gel for purity and ELISAs for binding, see results. A rabbit antibody 11B that targets 241 glycan hole (BG505 specific, catalog U90R79) does not to bind to this protein (ref: Cell Rep. 2016;16(9):2327-38.).

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