| 产品名称 | ReVacc Scientific U97O74 | MERS-CoV Trimeric Spike protein |
|---|---|
| 目录号 | U97O74 |
| 别名 | MERS-CoV Trimeric Spike protein |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| U97O74 | 100 μg; 500 μg | 咨询 | 咨询 |
ReVacc Scientific U97O74 | MERS-CoV Trimeric Spike protein
品名：MERS-CoV Trimeric Spike protein
货号：U97O74
品牌：ReVacc Scientific
规格：100 μg；500 μg
产品描述
| Source | HEK293 cells | Tag | His & Strep |
| Organism | MERS-Related Coronavirus (virus caused 2015 outbreak in Republic of Korea) | Biosafety Level | BSL-1 |
| Applications | Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others | ||
| Storage | Recommend -80°C storage. Store at 4°C for short-term only. Multiple freeze/Thaw cycles will reduce its activity. | ||
A recombinant trimeric form of the spike glycoprotein (S protein) of MERS-Cov (GenBank: JX869059.2) was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (V1060P and L1061P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition and Native blue gels, with results proving trimerization and glycosylation. We also evaluated the binding of one MERS Positive control mAb-RV45 and published MERS27 antibody in ELISAs, see results.
