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产品名称 ReVacc Scientific Y58C40 | Monkeypox Virus Trimeric A29 protein
目录号 Y58C40
别名 Monkeypox Virus Trimeric A29 protein
外观 见COA
分子量 N/A
CAS N/A
溶解度
存储条件 见COA
保存时间 见COA
备注1
备注2
目录号 规格 价格 库存状态  
Y58C40 100 μg; 500 μg 咨询 咨询


ReVacc Scientific Y58C40 | Monkeypox Virus Trimeric A29 protein


品名:Monkeypox Virus Trimeric A29 protein

货号:Y58C40

品牌:ReVacc Scientific

规格:100 μg;500 μg


产品描述

SourceInsect cellsTagHis
OrganismMonkeypox virus 2022 StrainBiosafety LevelBSL-1
ApplicationsCrystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others
StorageRecommend -80°C storage. Store at 4°C for short-term only.  Multiple freeze/Thaw cycles will reduce its activity.


Monkeypox virus (MPXV) is a member of the Orthopoxvirus of the family Poxviridae. A27 protein is a target for viral neutralization and serves one of important components in vaccine. The native A27 is a homotrimeric extracellular protein that is attached to the viral membrane.  Monkeypox virus A29 protein binds to cell surface heparan similar to Vaccinia virus strain Copenhagen A27 protein.   A recombinant form of Monkeypox virus protein A29 (Genbank:  ON563414.3, MPXV_USA_2022_MA001; ON838178.1, isolate SI2022_S7) was produced by insect cells, followed by purification.  Amino acid sequence for A29 is dgtlfpgdddlaipateffstkaaknpetkreaivkaygddneetlkqrltnlekkitnittkfeqiekcckrndevlfrlenhaetlraamislakkidvqtgrhpye. This product contains trimerization domain and C-terminal 6 hexa-histidine tag with sequence available upon request. The monomeric form of this product is predicted to be a molecular mass of ~18 kDa. This product has been run on SDS-PAGE with reduced condition. We also evaluated its binding to anti-His antibody with a negative control. The trimeric conformation was evaluated with SEC (Superose 6 column) with two peaks that we assigned one as a trimer fraction peak.  The product is not a SEC fraction.

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