| 产品名称 | ReVacc Scientific S95L46 | Monkeypox Virus Dimeric A35R protein |
|---|---|
| 目录号 | S95L46 |
| 别名 | Monkeypox Virus Dimeric A35R protein |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| S95L46 | 100 μg; 500 μg | 咨询 | 咨询 |
ReVacc Scientific S95L46 | Monkeypox Virus Dimeric A35R protein
品名：Monkeypox Virus Dimeric A35R protein
货号：S95L46
品牌：ReVacc Scientific
规格：100 μg；500 μg
产品描述
| Source | Insect cells | Tag | His & Myc |
| Organism | Monkeypox virus 2022 Strain | Biosafety Level | BSL-1 |
| Applications | Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others | ||
| Storage | Recommend -80°C storage. Store at 4°C for short-term only. Multiple freeze/Thaw cycles will reduce its activity. | ||
Monkeypox virus (MPXV) is a member of the Orthopoxvirus of the family Poxviridae. A35R extracellular enveloped virus (EEV) envelope glycoprotein is needed for formation of actin-containing microvilli and cell-to-cell spread of virion EEV membrane phosphoglycoprotein, similar to Vaccinia virus strain Copenhagen A33R. A33 contains a membrane-proximal cysteine on ectodomain that forms an intermolecular disulfide bridge. A recombinant form of monkeypox virus protein A35R (Genbank: ON563414.3, MPXV_USA_2022_MA001; ON838178.1, isolate SI2022_S7) was produced by insect cells, followed by purification. This product contains ectodomain only (58-end), N-terminal Myc tag and C-terminal 6 hexa-histidine tag with amino acid sequence available upon request. The ectodomain includes cysteines for dimer formation. Amino acid sequence for A35R: lnqcmsankaaitdsavavaaassthrkvvssttqydhkescnglyyqgscyilhsdyksfedakancaaesstlpnksdvlttwlidyvedtwgsdgnpitkttsdyqdsdvsqevrkyfct. The monomeric form of this product is predicted to be a molecular mass of ~17 kDa. This product has been run on SDS-PAGE with reduced condition. We also evaluated its binding to antibodies of anti-His, anti-Myc or clone RV10. The dimeric conformation was evaluated with SEC (Superose 6 column) with two peaks that we assigned one as a dimer fraction peak. The product is not a SEC fraction.
