| 产品名称 | ReVacc Scientific J23V36 | SARS-CoV-2 Trimeric Spike protein D614G (D614 mutant) |
|---|---|
| 目录号 | J23V36 |
| 别名 | SARS-CoV-2 Trimeric Spike protein D614G (D614 mutant) |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| J23V36 | 100 μg; 500 μg | 咨询 | 咨询 |
ReVacc Scientific J23V36 | SARS-CoV-2 Trimeric Spike protein D614G (D614 mutant)
品名：SARS-CoV-2 Trimeric Spike protein D614G (D614 mutant)
货号：J23V36
品牌：ReVacc Scientific
规格：100 μg；500 μg
产品描述
| Source | HEK293 cells | Tag | His & Strep |
| Organism | SARS-Related Coronavirus 2 (virus caused COVID-19) | Biosafety Level | BSL-1 |
| Applications | Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others | ||
| Storage | Recommend -80°C storage. Store at 4°C for short-term only. Multiple freeze/Thaw cycles will reduce its activity. | ||
A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov-2 (GenBank: MN908947) bearing a mutated S protein from D (Asp) to G (Gly) at site of 614 was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (N1067P and L1068P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition and Native blue gels as well as SEC, with results proving trimerization and glycosylation. We also evaluated the binding of two SARS-CoV-2 Positive control mAbs and CR3022 antibody in ELISAs, see results.
