| 产品名称 | ReVacc Scientific R12T26 | SARS-CoV-2 Trimeric Spike protein B.1.351 (Beta variant) |
|---|---|
| 目录号 | R12T26 |
| 别名 | SARS-CoV-2 Trimeric Spike protein B.1.351 (Beta variant) |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| R12T26 | 100 μg; 500 μg | 咨询 | 咨询 |
ReVacc Scientific R12T26 | SARS-CoV-2 Trimeric Spike protein B.1.351 (Beta variant)
品名：SARS-CoV-2 Trimeric Spike protein B.1.351 (Beta variant)
货号：R12T26
品牌：ReVacc Scientific
规格：100 μg；500 μg
产品描述
| Source | HEK293 cells | Tag | His & Strep |
| Organism | SARS-Related Coronavirus 2 (virus caused COVID-19) | Biosafety Level | BSL-1 |
| Applications | Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe and others | ||
| Storage | Recommend -80°C storage. Store at 4°C for short-term only. Multiple freeze/Thaw cycles will reduce its activity. | ||
A recombinant trimeric form of the spike glycoprotein (S protein) of SARS-Cov-2 (GenBank: MN908947) bearing mutations of L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V identified in Beta variant, was produced by human embryonic kidney HEK293 cells, followed by purification. This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (N1067P and L1068P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling. Amino acid sequence is available upon request. This product has been run on SDS-PAGE with reduced condition with result showing pure and expected size. We also evaluated the binding of two SARS-CoV-2 control mAbs (RV57 and RV94), our S2 subunit-specific RV82 and CR3022 antibody in ELISAs, see results. Two antibodies targeting NTD or RBD lost binding to this spike, compared with WT spike, as all mutations occur within NTD or RBD .
