| 产品名称 | ReVacc Scientific W30Y61 | Human CD8 Alpha&Beta (CD8A&CD8B) Heterodimer Protein |
|---|---|
| 目录号 | W30Y61 |
| 别名 | Human CD8 Alpha&Beta (CD8A&CD8B) Heterodimer Protein |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| W30Y61 | 100 μg; 500 μg | 咨询 | 咨询 |
ReVacc Scientific W30Y61 | Human CD8 Alpha&Beta (CD8A&CD8B) Heterodimer Protein
品名：Human CD8 Alpha&Beta (CD8A&CD8B) Heterodimer Protein
货号：W30Y61
品牌：ReVacc Scientific
规格：100 μg；500 μg
产品描述
| Source | Human embryonic kidney HEK cells | Tag | His, Myc and Strep |
| Organism | - | Biosafety Level | - |
| Applications | Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays | ||
| Storage | Recommend -80°C storage. Store at 4°C for short-term only. Multiple freeze/Thaw cycles will reduce its activity. | ||
Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein was produced by human embryonic kidney HEK cells, followed by purification. CD8 alpha (CD8A ectodomain, Ser 22 - Asp 182, Accession: P01732-1) was fused with a domain called “key domain” (proprietary technology), followed with HRV3C cleavage and Myc and Twin-Strep tags at the C-terminus. CD8 beta (CD8B ectodomain, Leu 22 - Pro 170, Accession: P10966-1) was fused with a domain called “lock domain” (proprietary technology), followed with HRV3C cleavage and 6 hexa-histidine tag at the C-terminus. The purity has been examined by reducing SDS-PAGE, where protein migrates ~62 kDa. We evaluated its binding to anti-His tag antibody (clone RV7, Cat.No. J96C86), anti-Myc tag antibody (clone RV19, Cat.No. M52T82), and a human CD8A-specific antibody (clone RV65, Cat.No. U18G96) in ELISAs. This protein simultaneously binding to His and Myc tag-specific antibodies validates the heterodimeric conformation. The protein can be cleaved using HRV3C for tag-free.
