ReVacc Scientific M32H50 | VSV-Pseudovirus_SARS-CoV-2 D614G (Spike D614G mutant) Spike with luciferase reporter

品名：VSV-Pseudovirus_SARS-CoV-2 D614G (Spike D614G mutant) Spike with luciferase reporter

货号：M32H50

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：SARS-Related Coronavirus 2 (virus caused COVID-19)

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry a mutated Spike (S) protein from D (Asp) to G (Gly) at site of 614 of SARS-CoV-2 (GenBank: MN908947), which was designed by codon optimizing the S sequence for mammalian expression, and had 18-aa cytoplasmic tail truncation for optimal infection. D614G mutant was found to be prevalent from April 2020 and enhances viral transmission (Cell 182, 1-16; 2020). As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing the undiluted pseudovirus (red) generates signal 100,000-fold higher than uninfected control (cell alone as background in blue), and 10-fold higher than WT pseudovirus (green). We also evaluated the neutralizing activity of one SARS-CoV-2 Positive control mAb by using this pseudotyped virus, see results.