ReVacc Scientific G99O57 | VSV-Pseudovirus_SARS-CoV-2 B.1.351 (Beta strain) Spike with luciferase reporter

品名：VSV-Pseudovirus_SARS-CoV-2 B.1.351 (Beta strain) Spike with luciferase reporter

货号：G99O57

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：SARS-Related Coronavirus 2 (virus caused COVID-19), B.1.351 variant first identified in South Africa

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-Cov-2 (GenBank: MN908947) with multiple mutations in following: L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing the undiluted pseudovirus (green) generates signal ~10,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of two mAbs (RV57 and RV88) by using this pseudotyped virus, see results.