ReVacc Scientific D33Z75 | VSV-Pseudovirus_SARS-CoV-2 B.1.617.2 (Delta strain) Spike with luciferase reporter

品名：VSV-Pseudovirus_SARS-CoV-2 B.1.617.2 (Delta strain) Spike with luciferase reporter

货号：D33Z75

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：SARS-Related Coronavirus 2 (virus caused COVID-19), B.1.617.2 variant (Delta strain) first identified in India

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), including: T19R, K77R, G142D, Δ156-157, R158G, A222V, L452R, T478K, D614G, P681R, and D950N. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing the undiluted pseudovirus generates signal over 10,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of one published control mAb (Science, 2020: 369, p643) by using this pseudotyped virus, see results.