ReVacc Scientific N64K21 | VSV-Pseudovirus_SARS-CoV-2 B.1.1.529 (Omicron Strain) Spike with luciferase reporter

品名：VSV-Pseudovirus_SARS-CoV-2 B.1.1.529 (Omicron Strain) Spike with luciferase reporter

货号：N64K21

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：SARS-Related Coronavirus 2 (virus caused COVID-19), Omicron variant (B.1.1.529 strain) first identified in South Africa.

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron (B.1.1.529) (BA.1), including: A67V, Δ69-70, T95I, G142D/Δ143-145, Δ211/L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing the undiluted pseudovirus generates signal over 10,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of two published control mAbs by using this pseudotyped virus with one losing neutralizing activity against this variant.