ReVacc Scientific T53L53 | VSV-Pseudovirus_MERS-CoV Spike with luciferase reporter

品名：VSV-Pseudovirus_MERS-CoV Spike with luciferase reporter

货号：T53L53

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：Middle East respiratory syndrome coronavirus (MERS-CoV), the virus caused 2015 outbreak in Republic of Korea.

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry S protein of Middle East respiratory syndrome coronavirus (MERS-CoV, GenBank No. JX869059.2), which was designed by codon optimizing the S sequence for mammalian expression, and had 16-aa cytoplasmic tail truncation for optimal infection. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing the undiluted pseudovirus (green) generates signal 100,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of our MERS Positive control mAb and one published mAb called MERS27 by using this pseudotyped virus, see results.