ReVacc Scientific V44X39 | VSV-Pseudovirus_SARS-CoV (2003 strain) Spike with luciferase reporter

品名：VSV-Pseudovirus_SARS-CoV (2003 strain) Spike with luciferase reporter

货号：V44X39

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：SARS-Related Coronavirus (virus caused 2003 outbreak)

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry Spike (S) protein of SARS-Cov (Severe Acute Respiratory Syndrome, GenBank: AY278554.2), which was designed by codon optimizing the S sequence for mammalian expression, and had 28-aa cytoplasmic tail truncation for optimal infection. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing the undiluted pseudovirus (green) generates signal 100,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of our SARS Positive control mAb and one published mAb called 80R by using this pseudotyped virus, see results.