ReVacc Scientific M85H4 | VSV-Pseudovirus Lassa Virus Glycoprotein Complex with Luciferase Reporter (Josiah Strain)

品名：VSV-Pseudovirus Lassa Virus Glycoprotein Complex with Luciferase Reporter (Josiah Strain)

货号：M85H4

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：Lassa virus (LASV), virus caused Lassa fever.

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry glycoprotein complex (GPC) of Lassa virus (LASV) Josiah Strain (GenBank: P08669). LASV causes Lassa fever that is a severe hemorrhagic manifestations. GPC including GP1, GP2 and an unusual stable signal peptide (SSP) is responsible for receptor binding and fusion of the virion with a cell membrane. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing that the starting 2-fold diluted pseudovirus generates signal >100,000-fold higher than uninfected control (cell alone as background in blue). We also evaluated the neutralizing activity of a neutralizing antibody RV32 (Catalog # M17T52) with a negative control by using this pseudotyped virus. The results showed the neutralization titer for RV32 is 1 ng/ml. See figure