ReVacc Scientific W95K98 | Pseudovirus_HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

品名：Pseudovirus_HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

货号：W95K98

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：Human metapneumovirus (HMPV) isolate NL/1/00/A1

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length fusion (F) protein of Human metapneumovirus (HMPV) isolate NL/1/00/A1 (294E). HMPV is a respiratory virus with fusion (F) protein as the sole target of neutralizing antibodies. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing that the starting 2-fold diluted pseudovirus generates signal ~ 10,000-fold higher than uninfected control (cell alone as background in blue). An RSV and hMPV cross-reactive mAb RV35, along with a known neutralizing mAb (D25) were used to validate its neutralizing activity against this pseudovirus. See picture.