ReVacc Scientific P11A48 | Pseudovirus_RSV Fusion with Luciferase Reporter (A2 strain)

品名：Pseudovirus_RSV Fusion with Luciferase Reporter (A2 strain)

货号：P11A48

品牌：ReVacc Scientific

规格：200 μl; 1000 μl

产品描述

Organism：Human respiratory syncytial virus (RSV) isolate A2 strain

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full-length fusion (F) protein of Human respiratory syncytial virus (RSV) isolate A2 strain (UniProtKB/Swiss-Prot: P03420.1, with prefusion-stabilizing mutations). RSV is a respiratory virus with fusion (F) protein as the major target of neutralizing antibodies/vaccine-elicited sera. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See our titration result showing that the starting 2-fold diluted pseudovirus generates signal ~ 10,000-fold higher than uninfected control (cell alone as background in blue). Three known neutralizing mAbs (D25, 101F and MPE8) were used to validate its neutralizing activity against this pseudovirus. See picture.