Trialtusbioscience | CRISPR-Cas9 Protein | 40-1320; 40-1325

品名：CRISPR-Cas9 Protein

货号：40-1320; 40-1325

规格：100 μg; 500 μg

品牌：Trialtusbioscience

产品描述

TriAltus' highly pure, tag-free Cas9 protein is used for genome editing with CRISPR technology. Recombinant Streptococcus pyogenes Cas9 (wild-type) protein is expressed in Escherichia coli and features two nuclear localization signals (NLS), one at the N-terminus and another at the C-terminus. Cas9 protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables efficient and precise genome editing in cells.

PURITY：~99% (SDS-PAGE gel analysis)

ENZYME SOURCE：Streptococcus pyogenes Cas9 is expressed in E. coli with two (N-terminal and C-terminal) nuclear localization signals (NLS), and is purified with our CL7 tag technology.

STORAGE/SHIPPING CONCENTRATION：5 mg/mL

STORAGE BUFFER：10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol

RECOMMENDED STORAGE CONDITIONS：-80°C

EXPIRATION：6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.