品名：Seraseq® MSI Reference Panel Mix AF5% 

货号：0710-1675 

品牌：SeraCare 

Product Specifications

 # of variants 5 

Allele Frequency 5% 

Concentration 20 ng/µL 

Fill Size 15 µl 

Total DNA 300 ng 

Number of vials 2 (tumor-normal matched) 

Matrix TE Buffer 

Marker                     Gene                         Chromosome               Position (hg19 based)              Comment 

BAT-25                KIT (intron16)                      chr4                            55598211                             25T->19T 

BAT-26               MSH2 (intron5)                     chr2                            47641559                             27A -> 17A 

NR-21                SLC7A8 (5’UTR)                  chr14                          23652346                             21A -> 13A 

NR-24                ZNF2 (3’UTR)                      chr2                            95849361                             23T -> 17T 

MONO-27¹      MAP4K3 (intron 3)                  chr2                            39573062                              27A -> 21A 

MONO-27^{1       MAP4K3 (intron13)                 chr2                            39536690                              27A -> 21A}

¹There is ambiguity in the literature on the MONO-27 locus so two constructs are included in the product to ensure 

compatibility (see Bacher J, Halberg R,  Kent-First M, Wood KV. “Methods and kits for detecting mutations” US 

Patent US20090068646A1 issued March 12, 2009; and Pino MS, Chung DC. “Application of molecular diagnostics 

for the detection of Lynch syndrome.” Expert review of molecular diagnostics vol. 10,5 (2010): 651-65. doi:10.1586/

erm.10.45).

Details

Microsatellites are regions of DNA repeats with different lengths, i.e., instability, highlighting DNA mismatch repair 

gene deficiencies. Typical lengths for these DNA repeats are between 1-6 base pairs and vary person to person 

such that each person has a set length of these microsatellites in their genome. Measurements of MSI have 

traditionally been performed using qPCR/CE analysis methods, but new methodologies are now available that 

includes digital droplet PCR (ddPCR) as well as Next Generation Sequencing (NGS). Microsatellite instability status 

has been linked to favorable outcomes in I-O treatment response by patients with diseases such as Lynch Syndrome 

and colorectal cancer. Hence MSI measurements have become an important biomarker in immuno-oncology 

therapeutics. 

LGC SeraCare has developed microsatellite instability (MSI) reference materials containing the key mono- and 

dinucleotide biomarkers typically analyzed in molecular MSI assays – NR-21, NR-24, BAT-25, BAT-26 and MONO-27. 

These markers are blended at two different allele frequency levels – AF5% and AF20% - to support MSI assay LoD 

determinations and accurate MSI detection. These products are offered as tumor-normal matched pairs, with 

variants precisely quantitated by digital PCR as well as qPCR/CE against a highly characterized genomic DNA from 

a background WT cell line (GM24385) determined as microsatellite stable (MSS). 

FEATURES AND BENEFITS 

Plasmid-based spike-ins of 5 key MSI biomarkers typically analyzed in molecular assays that measure MSI in cancer 

patent samples 

Offered as tumor-normal matched pairs per AF level 

Each biomarker blended at two AF levels – 5% and 20% - to support assay LoD determinations and accurate MSI 

detection in patient samples 

Variant AFs quantitated by ddPCR and qPCR assays Normal background DNA is a highly characterized GM24385 

human genomic DNA known to be microsatellite stable (MSS) 

Manufactured within cGMP compliant and ISO 13485 certified facilities 

维百奥生物代理SeraCare对照及参比物质。

维百奥（北京）生物科技有限公司，自2018年与SeraCare（现隶属于LGC Clinical Diagnostics）合作以来，长期为中国客户

提供包括SeraSeq系列参比物质，领域包括肿瘤、生殖健康和遗传病。SeraCare研发和生产一系列临床参比物质，可以用于任何

研发阶段，以确保临床基因组学检测结果的准确性。

SeraCare参比物质涵盖的范围：

1）Next Generation Sequencing

2）Sanger Sequencing

3）Real-time PCR and digital PCR (dPCR)

4）Microarray

主要特点

1）Ready-to-use reference materials covering clinically-relevant variants and all variant types - SNVs, INDELS, CNVs, 

and RNA fusions

2）Highly multiplexed - provide significantly more data per NGS run, saving sequencing costs

3）Available in multiple formats to suite different workflow needs - purified DNA & RNA, ctDNA, encapsulated ctDNA 

in plasma, and FFPE

4）Manufactured in cGMP-compliant, ISO 13485-certified facilities

5）Stringent product release testing - all variants qualified by dPCR for allele frequencies or copy numbers