品名：Seraseq® FFPE Tumor Fusion RNA v4 Reference Material

货号：0710-0496 

品牌：SeraCare 

Product Specifications 

# of Fusions：18 

Size：10 µm 

Format：FFPE 

RNAYield*：>400 ng 

*From 1x10 µm FFPE curl using QIAGEN AllPrep DNA/RNA Prep kit/Qubit RNA HS quant

Details

As the diagnostic testing paradigm for chromosomal translocations and other complex structural variants continues 

to shift from single-site Fluorescent In Situ Hybridization (FISH) and targeted RT-PCR to highly multiplexed 

next-generation sequencing (NGS) assays, there is increasing need for highly multiplexed reference materials for these 

difficult mutations. 

The Seraseq FFPE Tumor Fusion RNA Reference Material v4 contains 18 clinically significant RNA fusions which are 

prevalent across different types of solid tumors, in an efficient single-sample format. RNA transcripts carrying 

structural variants are engineered into well-characterized wild-type cells. Cells are formalin-fixed and embedded in 

paraffin, and the FFPE block is cut into 10-micron sections, each of which typically yields >400 ng of purified total 

RNA. The transcript level for each fusion is quantified using highly sensitive digital PCR to establish ground-truth 

(and orthogonally validated with NGS), which helps ensure the material remains constant over time. 

    18 clinically relevant fusions evaluate assay sensitivity and specificity for important diagnostic, prognostic, and 

therapeutic targets 

    Full-process FFPE format may be treated like a clinical specimen, which can reveal pre-analytic variability 

    Quantification of transcript levels by dPCR establishes ground-truth and simplifies dilutions for Limit of Detection 

(LoD) experiments 

    Well-characterized GM24385 human genomic DNA as background wild-type material 

    Manufactured in GMP-compliant and ISO 13485-certified facilities  

For research use only. Not for use in diagnostic procedures. 

维百奥生物代理SeraCare对照及参比物质。

维百奥（北京）生物科技有限公司，自2018年与SeraCare（现隶属于LGC Clinical Diagnostics）合作以来，长期为中国客户

提供包括SeraSeq系列参比物质，领域包括肿瘤、生殖健康和遗传病。SeraCare研发和生产一系列临床参比物质，可以用于任何

研发阶段，以确保临床基因组学检测结果的准确性。

SeraCare参比物质涵盖的范围：

1）Next Generation Sequencing

2）Sanger Sequencing

3）Real-time PCR and digital PCR (dPCR)

4）Microarray

主要特点

1）Ready-to-use reference materials covering clinically-relevant variants and all variant types - SNVs, INDELS, CNVs, 

and RNA fusions

2）Highly multiplexed - provide significantly more data per NGS run, saving sequencing costs

3）Available in multiple formats to suite different workflow needs - purified DNA & RNA, ctDNA, encapsulated ctDNA 

in plasma, and FFPE

4）Manufactured in cGMP-compliant, ISO 13485-certified facilities

5）Stringent product release testing - all variants qualified by dPCR for allele frequencies or copy numbers