Ubiqbio | UbiQ-071 | Cy5-Ub-VME

品名：Cy5-Ub-VME

货号：UbiQ-071

规格：50 µg

品牌：Ubiqbio

产品描述

a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), labeled with a Cy5 dye on the N-terminus

Cy5-Ub-VME (UbiQ-071) is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), which is labeled on the N-terminus with a Cy5 dye (Cy5, exc 625-650 nm, abs 670 nm). This ubiquitin-based activity probe can be used for activity profiling experiments and the control of DUB inhibitor specificity. Whereas the first-generation activity probes (such as HA tagged Ub-VME) require immunoblotting for detection. This probe allows for detection of DUB labeling by in-gel fluorescence. This direct and more sensitive read-out gives more distinct labeling patterns than can be achieved by immunoblotting. In addition, cross-reactivity of antibodies can lead to background labeling, something that is not observed with UbiQ-071.

Applications: Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling

Category: activity-based probes