Ubiqbio | UbiQ-073 | ISG15-FP

品名：ISG15-FP

货号：UbiQ-073

规格：50 µg

品牌：Ubiqbio

产品描述

a fluorescence polarization assay reagent for deISGylating enzymes, based on a TAMRA modified Lys-Gly sequence, linked to mouse ISG15

UbiQ-073 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to (mouse) ISG15 (Interferon stimulated gene 15) via a native isopeptide bond with the lysine side-chain. ISG15 is a ubiquitin-like protein whose expression is increased following stimulation with type 1 Interferons. A typical substrate concentration is 100 nM. Effective concentrations of deISGylating enzymes depends on specific assay conditions and method of detection. UbiQ-073 is effectively processed by the known deISGylating enzyme USP18.

Applications: DUB activity profiling, Compound library screening

Category: activity assay reagents