Ubiqbio | UbiQ-076 | Biotin-Ahx-Ub-PA

品名：Biotin-Ahx-Ub-PA

货号：UbiQ-076

规格：50 µg

品牌：Ubiqbio

产品描述

a potent and specific inhibitor of deubiquitylating enzymes (DUBs), labeled on the N-terminus with biotin and a C-terminal propargylamide (PA) electrophile.

Biotin-Ahx-Ub-PA (UbiQ-076) is a potent and specific inhibitor of deubiquitylating enzymes (DUBs), which is labeled on the N-terminus with biotin and contains a C-terminal propargylamide (PA) electrophile. An aminohexanoic acid linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. The C-terminal propargylamide (PA) electrophile has two characteristics: 1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. 2. it targets all three major DUB families: UCH, USP and OTU.

Applications: Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling

Category: activity-based probes