Ubiqbio | UbiQ-078 | HA-Ahx-Ahx-Ub-PA

品名：HA-Ahx-Ahx-Ub-PA

货号：UbiQ-078

规格：50 µg

品牌：Ubiqbio

产品描述

a potent and specific inhibitor of deubiquitylating enzymes (DUBs) containing the propargylamide (PA) warhead

UbiQ-078 (HA-Ahx-Ahx-Ub-PA) is a potent and specific inhibitor of deubiquitylating enzymes (DUBs) containing the propargylamide (PA) as a newly discovered DUB activity warhead. It can be used for activity profiling experiments and determining DUB inhibitor specificity, using two unique capabilities of the PA warhead: 1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. 2. it targets all three major DUB families: UCH, USP and OTU. UbiQ-078 is N-terminally tagged with the HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification of DUBs by anti-HA antibodies and/or anti-HA-agarose. The HA tag is separated from the Ubiquitin N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag.

Applications: Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling

Category: activity-based probes