Ubiqbio | UbiQ-102 | Biotin-Ahx-Ub-Dha

品名：Biotin-Ahx-Ub-Dha

货号：UbiQ-102

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with biotin on the N-terminus

Biotin-Ahx-Ub-Dha (UbiQ-102) is an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-102 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond.

Applications: Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling, Target inhibitor profiling

Category: activity-based probes