Ubiqbio | UbiQ-103 | His6-Ahx-Ahx-Ub-Dha

品名：His6-Ahx-Ahx-Ub-Dha

货号：UbiQ-103

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with His6 on the N-terminus

His6-Ahx-Ahx-Ub-Dha (UbiQ-103) is an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with a His6 tag; two aminohexanoic acid (Ahx) linkers are used to create extra space between the His6 and Ub protein for efficient access of His6 binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of His6 site. UbiQ-103 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond.

Applications: Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling, Target inhibitor profiling

Category: activity-based probes