Ubiqbio | UbiQ-104 | Cy5-Ub-Dha

品名：Cy5-Ub-Dha

货号：UbiQ-104

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with a Cy5 dye on the N-terminus

Cy5-Ub-Dha (UbiQ-104) is an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with a Cy5 tag. It has been prepared by total chemical synthesis and is therefore well-defined in terms of Cy5 site. UbiQ-104 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond.

Applications: Phenotypic protein profiling, Compound library screening, Target inhibitor profiling

Category: activity-based probes