Ubiqbio | UbiQ-119 | (Biotin-Ahx-Ub)-(Cys-Ahx-Ub) K48 linked

品名：(Biotin-Ahx-Ub)-(Cys-Ahx-Ub) K48 linked

货号：UbiQ-119

规格：50 µg

品牌：Ubiqbio

产品描述

a native K48 linked di-ubiquitin, modified with biotin on the N-terminus of the distal Ub and cysteine on the N-terminus of the proximal Ub

Background. UbiQ-119 is a native K48 linked di-ubiquitin which is modified with a biotin tag on the distal Ub and an N-terminal cysteine on the proximal Ub.* The 6-aminohexanoic acid (Ahx) linker is used to create extra space for efficient access of biotin binding entities and Cys reactive reagents. The biotin can be used as conjugation site for labeled streptavidin or anti-biotin antibodies. The Cys residue can be modified by two methods: 1) by thiol alkylation with thiol-reactive moieties (such as maleimides and iodoacetamides) 2) by native chemical ligation using activated esters (such as thioesters and NHS esters). Ligation via method 2 retains the thiol group of the Cys residue which could then be used for attaching another label if desired. Overall, UbiQ-119 is designed to allow for the creation of various K48 diUb based conjugates which could serve for example as (TR-FRET/FRET) DUB assay reagents.

Applications: DUB activity profiling, Compound library screening

Category: activity assay reagents