Ubiqbio | UbiQ-121 | Biotin-Ahx-Ub(1-75)-Dha-Ub

品名：Biotin-Ahx-Ub(1-75)-Dha-Ub

货号：UbiQ-121

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe based on linear diubiquitin, labeled with biotin on the N-terminus

Biotin-Ahx-Ub(1-75)-Dha-Ub (UbiQ-121) is an activity-based probe for the deubiquitinating enzyme (DUB) OTULIN. It is based on linear diUb in which the Gly76-Met1 dipeptide is replaced by a dehydroalanine-Met (Dha-Met) dipeptide. The N-terminus of the distal Ub is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space for efficient access of biotin binding entities. UbiQ-121 has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-121 reacts with OTULIN in a covalent manner without being cleaved into monoUb, indicating correct positioning of the Dha electrophile for reacting with the nucleophilic active site cysteine of OTULIN.

Applications: Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling

Category: activity-based probes