Ubiqbio | UbiQ-122 | 5-carboxyRh110-Nedd8-Dha

品名：5-carboxyRh110-Nedd8-Dha

货号：UbiQ-122

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for Nedd8 E1, E2 and E3 enzymes, labeled with cRh110 on the N-terminus

5-carboxyRh110-Nedd8-Dha (UbiQ-122) is an activity-based probe for Nedd8 E1, E2 and E3 ligases. It is based on the Nedd8 sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the green fluorescent 5-carboxyrhodamine110 dye (cRh110). It has been prepared by total chemical synthesis and is therefore well-defined in terms of dye site. UbiQ-122 is processed in a native manner by E1, E2 and E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzyme, thereby creating a covalent bond.

Applications: Phenotypic protein profiling, Compound library screening, Target inhibitor profiling

Category: activity-based probes