Ubiqbio | UbiQ-124 | His10-SUMO3-Dha

品名：His10-SUMO3-Dha

货号：UbiQ-124

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for the SUMO ligation pathway, based on SUMO3 K11R C47S labeled with His10 on the N-terminus

UbiQ-124 is an activity-based probe for the SUMO ligation pathway. It is based on the SUMO3 sequence in which the C-terminal Gly has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the His10 affinity tag and the SUMO3 sequence contains a K11R (to prevent polysumoylation) and C47S mutation. UbiQ-124 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond.

Applications: Crystallization, Western Blot

Category: activity-based probes