Ubiqbio | UbiQ-131 | 5-carboxyRh110-Ub-Dha

品名：5-carboxyRh110-Ub-Dha

货号：UbiQ-131

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with cRh110 on the N-terminus

UbiQ-131 is an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the green fluorescent 5-carboxyrhodamine110 dye (cRh110). It has been prepared by total chemical synthesis and is therefore well-defined in terms of dye site. UbiQ-131 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond.

Applications: Phenotypic protein profiling, Compound library screening, Target inhibitor profiling

Category: activity-based probes