Ubiqbio | UbiQ-154 | Ub photoLeu73

品名：Ub photoLeu73

货号：UbiQ-154

规格：100 µg

品牌：Ubiqbio

产品描述

a crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu)

UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu). Upon irradiation of the diazirine with UV light, a very reactive carbene species is formed. Unspecific binding is minimal for carbene based crosslinking because carbenes are quenched quickly by water, meaning only residues that are very nearby (and thereby contribute to tight binding) will react covalently. Thus any unbound UbiQ-154 will react with water before being able to undergo non-specific reactions with other proteins.

Applications: Crystallization, Pull down, Western Blot, MS

Category: activity assay reagents