Ubiqbio | UbiQ-158 | His6-3C-MAP1LC3a-Dha

品名：His6-3C-MAP1LC3a-Dha

货号：UbiQ-158

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively), based on MAP1LC3a C17S labeled with His6 on the…

UbiQ-158 (His6-3C-MAP1LC3a-Dha) is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). UbiQ-158 is based on the MAP1LC3a protein sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue (Dha). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S). UbiQ-158 is processed in a native manner by E1-E2-E3 enzymes that recognize MAP1LC3a and during this process it forms an electrophilic intermediate that can react with an active-site Cys residue in the E1-E2-E3 cascade, thereby creating a covalent bond.

Applications: Crystallization, Western Blot

Category: activity-based probes