Ubiqbio | UbiQ-193 | HA-Ahx-Ahx-Ub-VPS

品名：HA-Ahx-Ahx-Ub-VPS

货号：UbiQ-193

规格：50 µg

品牌：Ubiqbio

产品描述

an activity-based probe for deubiquitinating enzymes (DUBs), labeled with HA on the N-terminus

HA-Ahx-Ahx-Ub-VPS (UbiQ-193) is an irreversible inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It contains a vinyl pentynyl sulfone (VPS) warhead and N-terminal HA-tag (YPYDVPDYA). The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. The VPS electrophile allows for post-labeling modification of covalent DUB::UbiQ-193 complexes by using click chemistry with for example biotin-azide.

Applications: Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling

Category: activity-based probes