Ubiqbio | UbiQ-237 | His6-Ahx-Ahx-SUMO2-VPS

品名：His6-Ahx-Ahx-SUMO2-VPS

货号：UbiQ-237

规格：50 µg

品牌：Ubiqbio

产品描述

a potent, irreversible and specific activity-based probe for SUMO proteases, labeled with His6 on the N-terminus

His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases that is prepared by total chemical synthesis. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile (Figure 1) and an N-terminal His6 sequence which allows for sensitive identification or purification by anti-His6 antibodies and/or anti-His6-agarose. The VPS electrophile allows for post-labeling modification of cross-linked UbiQ-237::SENP complexes by using click chemistry with for example biotin-azide.

Applications: Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling

Category: activity-based probes