Ubiqbio | UbiQ-L06 | DUB activity-based probe mix – HA tagged

品名：DUB activity-based probe mix – HA tagged

货号：UbiQ-L06

规格：panel

品牌：Ubiqbio

产品描述

UbiQ-L06 is a panel of three activity-based probes for deubiquitinating enzymes (DUBs) (50 μg each).

UbiQ-L06 is a panel of three activity-based probes for deubiquitinating enzymes (DUBs) (50 μg each). The probes are based on ubiquitin and labeled on the N-terminus with a HA-tag. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies and anti-HA-agarose. The HA tag is separated from the Ub N-terminus by two 6-aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. By mixing the three probes, a highly DUB reactive probe mix is obtained that contains the combined reactivity of the PA (propargyl amide), VME (vinyl methyl ester) and VS (vinyl sulfone) electrophiles. As a result the labelling efficiency of the mix is higher than using each probe separately.

Applications: Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling

Category: activity-based probes, explorer panels