Gene Tools, LLC produces and sells Morpholino antisense oligos specifically designed for gene regulation in scientific research and drug development. The company is comprised of several senior scientists with Ph.D. degrees, offering free technical consultation and Morpholino sequence design services to customers worldwide. Morpholinos, recognized as the premier tool for gene regulation research, are widely used for: blocking target mRNA translation (gene knockdown), inducing alternative RNA splicing (gene knockdown or upregulation), blocking miRNA, inhibiting viral RNA, and blocking non-coding RNA.

■ Morpholino Services

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Morpholinos belong to the third generation of antisense oligonucleotides, exclusively developed and synthesized by Gene Tools. Morpholinos primarily inhibit target gene function by blocking the protein translation process. They are widely used in developmental biology and cellular research and have demonstrated excellent experimental performance.

Morpholinos are built on a morpholine ring backbone, and this unique structural property provides several advantages:

•Compared with other antisense oligonucleotides, Morpholinos exhibit strong resistance to nuclease degradation.

•After binding to target sequences, Morpholinos act through steric hindrance, without activating RNase H, and therefore do not induce degradation of the target mRNA.

•Morpholinos designed against the translation start site inhibit protein translation, while those targeting pre-mRNA splice sites can alter RNA splicing, generating different transcripts. This makes them highly useful for functional studies of gene domains and mutation analysis.

•They have strong binding affinity to target sequences, can penetrate secondary structures of mRNA, and provide high specificity.

To support Morpholino applications, Gene Tools has also developed specialized delivery systems that can safely and efficiently transport Morpholinos into the cytoplasm. As a result, Morpholinos are widely used in genetic research, drug target discovery, and validation.

In addition, Morpholinos can be modified with fluorescent labels, affinity tags, or reactive functional groups to meet different experimental requirements.

■ Product Portfolio

1. Custom Morpholino

Custom Morpholinos can be designed and synthesized according to the target gene sequence. They are commonly used in in vitro cell experiments or in microinjection models, such as zebrafish and Xenopus embryos.

2. Vivo-Morpholino

Vivo-Morpholinos are designed for in vivo animal models (including mice, rats, dogs, and primates), as well as cell culture and organ culture.

With special terminal modifications, Vivo-Morpholinos have enhanced cell membrane penetration, allowing efficient delivery into tissues. This makes systemic gene function studies in adult animals (particularly mice and rats) possible.

3. Prepared Control Oligos

Prepared Control Oligos are Morpholinos conjugated with cell-penetrating peptides synthesized for specific experimental requirements. They are suitable for in vivo animal models, cell culture, and organ culture systems.

4. Endo-Porter

To address the low uptake efficiency of Morpholinos in cultured cells, Gene Tools developed Endo-Porter, a specialized transfection reagent.

Endo-Porter promotes Morpholino delivery into the cytoplasm through a mild endosomal escape mechanism, significantly improving intracellular uptake.