EZ DNA Methylation-Direct Kit

Cat # Name                         Size

D5021 EZ DNA Methylation-Direct Kit 200 Rxns.

D5020 EZ DNA Methylation-Direct Kit 50 Rxns.

Brand：Zymo Research

_d5020_d5021_ez_dna_methylation-direct_kit.pdf

HIGHLIGHTS

Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.

Compatible with small sample inputs as few as 10 cells or 50 pg of DNA.

DNA recovered by this bisulfite conversion kit is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

DESCRIPTION

The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

TECHNICAL SRECIFICATIONS

Applications

Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.

Conversion

> 99.5%

Elution Volume

≥ 10 µl

Equipment

Thermocycler with heated lid and microcentrifuge.

Input

Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.

Processing Time

4 hours

Recovery

> 80%

Sample Source

Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.

Supplemental Info

Bisulfite Beginner Guide

Controls for DNA Methylation Assays

Bisulfite Conversion for Illumina Methylation Assays

Bisulfite Primer Design Tool Link