ZymoTaq qPCR Premix

Cat # Name                         Size

E2054 ZymoTaq qPCR Premix 50 Rxns

E2055 ZymoTaq qPCR Premix 200 Rxns

Brand ：Zymo Research

_e2054_e2055_zymotaq_qpremix.pdf

HIGHLIGHTS

Hot-start polymerase for easy setup at room temperature.

Robust amplification of DNA for genotyping, SNP, and HRM analysis.

Strong fluorescent signal for real-time and quantitative PCR assays.

DESCRIPTION

ZymoTaq qPCR PreMix contains all the reagents needed to perform quantitative PCR and other molecular downstream analysis such as high-resolution melt (HRM) analysis of DNA methylation and other general real-time PCR assays. This premix features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treated DNA. It also includes an intense dsDNA-specific fluorescent dye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits (E2005, E2006, E2007) for low copy detection of DNA from human, bacteria, or fungi.

TECHNICAL SRECIFICATIONS

Activity

5' - 3' DNA polymerization

Concentration

Reaction conditions at 1X (50 µl total volume) will contain 2 U of ZymoTaq DNA polymerase.

Equipment

Real-time PCR can be performed on any instrument that has optical scanners that do not require passive reference dye.

Storage

Store at ≤ -20°C for up to 12 months. Minimize exposure to light. Avoid repeated freeze/thawing of reagents.

Unit Definition

One unit (U) enzyme of ZymoTaq DNA Polymerase is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C.