All-In-One 5X RT MasterMix with gDNA Removal

G592

100 rxn

Cat. No. G592Print

Name All-In-One 5X RT MasterMix with gDNA Removal

Unit 100 rxn

Category Reverse Transcriptase & RT-PCR

Description

Enjoy accurate gDNA-free cDNA synthesis!

All-In-One 5X RT MasterMix with gDNA Removal is a convenient and ready-to-use formulation for first-strand cDNA synthesis,

 including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and 

this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will 

be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript Hot Reverse 

Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of 

Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream 

applications.®

Product Features:

Easy one-step setup reduces pipetting and sample handling

High cDNA yields from difficult samples with OneScript Hot Reverse Transcriptase (Cat. No. G593®)

Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)

Removes contaminating gDNA with temperature-sensitive DNase

Also includes dNTPs, Oligo (dT)s, and random primers

Earn 2X the rewards points

Enjoy accurate gDNA-free cDNA synthesis!

All-In-One 5X RT MasterMix with gDNA Removal is a convenient and ready-to-use formulation for first-strand cDNA synthesis, 

including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and 

this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will 

be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript Hot Reverse 

Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo

 (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream 

applications.®

Product Features:

Easy one-step setup reduces pipetting and sample handling

High cDNA yields from difficult samples with OneScript Hot Reverse Transcriptase (Cat. No. G593®)

Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)

Removes contaminating gDNA with temperature-sensitive DNase

Also includes dNTPs, Oligo (dT)s, and random primers

Earn 2X the rewards points

Request Free Sample

ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified

Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by 

Intertek (a MDSAP recognized auditing organization).

Product Component Quantity

All-In-One 5X RT MasterMix 100 rxn (400 µl)

Nuclease-Free H2O 1.0 ml

Application

Generation of templates for use in RT-PCR and qRT-PCR

cDNA synthesis from ssRNA

cDNA library construction

Generation of probes for hybridization

DNA primer extension

Concentration 5X

Storage Condition

Store at -20°C.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: 

Applied Biological Materials Inc, Cat. No. G592