Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit

GS313F

HyStem®-C

Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit

Catalog #GS312F #GS313F #GS1005F

HyStem-C Hydrogel Kit Thiolated Hyaluronic Acid

HyStem-C Hydrogel Kit Thiolated Hyaluronic Acid

HyStem-C Hydrogel Kit Thiolated Hyaluronic Acid

HyStem-C Hydrogel Kit Thiolated Hyaluronic Acid

HyStem®-C

Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit

Catalog #GS312F #GS313F #GS1005F

HyStem-C is a 3D hydrogel kit utilizing thiolated-hyaluronic acid (HA) and gelatin. HA is the simplest glycosaminoglycan

 (a negatively charged, linear, non-sulfated polysaccharide) that provides compression strength, lubrication, and hydration. 

HA regulates cell adhesion, motility, proliferation and differentiation.

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Product Description

 HyStem®-C Hydrogel Kits - The starter matrix.

HyStem®-C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully 

chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive

 crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S®). Gelin-S provides basic cell-attachment

 sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell

 types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM 

proteins to match native signals. 

Product may be shipped ambient, but please store components at the temperature stated on the vials until ready for use. 

Features

Hydrogels are suitable for culturing primary cells, stem cells and cell lines.

Cells can be encapsulated or grown on the hydrogel surface in any format, including culture flasks, 6- to 384-well plates or

 tissue culture inserts.

Hydrogels can be easily customized by the user to possess the desired stiffness and gelation time by manipulating component 

concentration and mixing ratios.

Customizable gelation properties including gelation time and hydrogel stiffness.

Gelation 

Reconstituted HyStem-C components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent,

 Extralink® (PEGDA) is added to a mixture of Glycosil®(thiol-modified hyaluronan) and Gelin-S® (thiol-modified gelatin). 

Gelation occurs in about twenty minutes after all three components are mixed. No steps depend on low temperatures or low

 pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase the gelation time. 

3D Cell Recovery Matrix 

For application where cell recovery is critical, the alternative crosslinker PEGSSDA is available for use with all HyStem, HyStem-C 

and HyStem-HP kits. This crosslinker provides the same advantages offered by Extralink with the additional benefit of containing

 easily reducible internal bonds. This allows for fast, easy recovery of single cells or clusters from the hydrogel for applications

 like RNA analysis or flow cytometry instead of slow enzymatic methods that can impact cell viability. Researchers are 

encouraged to contact us to determine the compatibility of particular cell types or culture systems with PEGSSDA.

Directions for Use

Download the HyStem®-C hydrogel kit instructions for:

2.5, 7.5 and 12.5 mL Kits

Note - You can store the entire kit at 4C or store the individual components at the temperatures indicated in the directions for

 use.