EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™

 CC-3162

Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176) required

 for growth of Endothelial Cells. Available for immediate shipment.

EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™

Catalog #: CC-3162

Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176) required

 for growth of Endothelial Cells. Available for immediate shipment.

Request Information or Quote or alternatively, generate the quote straight away by adding the product to the cart and simply 

clicking on “Generate Quote”.

Do you need a different format or volume for this media product? Contact Lonza CellBio Services to discuss custom format and

 formulation options.

Select Size

6 KITS

1 KIT

FGM™-2 Fibroblast Growth Medium-2 BulletKit™

CC-3132

Culture system contains FBMTM Basal Medium (CC-3131) and FGMTM-2 SingleQuotsTM supplements (CC-4126) required for

 growth of fibroblasts.

Product Overview

FGMTM-2 Fibroblast Growth Medium-2 was developed to support the growth of most primary human fibroblasts including

 lung and dermal fibroblasts. The kit contains basal medium and supplements packaged together for convenience to culture

 fibroblasts. FGMTM-2 Medium contains 2% serum.

Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit 

consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media

 (catalog no. CC-3132)  

Storage and Content

1 x FBMTM Basal Medium (CC-3131), 500 mL

1 x FGMTM-2 SingleQuotsTM Supplement Pack (CC-4126) containing:

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Gray Cap Vial with hFGF-B, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

1 x Bottle FBS, 10.00 mL

HCMTM Hepatocyte Culture Medium BulletKitTM

 CC-3198

Hepatocyte Culture Medium containing HBMTM Basal Medium (CC-3199) and HCMTM SingleQuot Supplements (CC-4182) 

required for growth of primary hepatocytes.

 Product Overview

The Hepatocyte Culture Medium BulletKitTM consists of the HBMTM Hepatocyte Basal Medium, 500 mL (CC-3199) and the

 HCMTM SingleQuotsTM Kit (CC-4182).

Once combinded together these reagents formulate a highly reproducible maintenance medium for primary hepatocytes from

 all species. The final medium is serum-free.

Storage and Content

1 x HBMTM (CC-3199) Bottle Basal Medium, 500 mL

1 x HCMTM (CC-4182) SinglequotsTM Supplement Pack containing:

1 x Transferrin, 0.50 mL

1 x Ascorbic Acid, 0.50 mL

1 x HEGF, 0.50 mL

1 x Insulin, 0.50 mL

1 x Hydrocortisone, 0.50 mL

1 x BSA (Fatty Acid Free), 10.5 mL

1 x GA-1000, 0.50 mL

REGM™ Renal Epithelial Cell Growth Medium BulletKit™

 CC-3190

Culture system containing REBMTM Basal Medium (CC-3191) and REGMTM SingleQuotsTM supplements (CC-4127) required 

for growth of Renal Epithelial Cells.

Product Overview

REGMTM Renal Epithelial Cell Growth Medium is formulated to maintain and expand most renal epithelial cells. REGMTM

 contains 0.5% serum.    

Select References: 

Diabetic Nephropathy is a chronic, progressive renal disease/diabetic kidney disease that develops in approximately one third 

of people with diabetes.  In a study, Lonza’s human proximal tubular epithelial cells were cultured in respective media 

(REGMTM). The group investigated the role of KLK1 (kallikrein) in tubular pro-inflammatory responses in cultured human RPTEC

 and examined the role of PAR-4 (Protease-Activated Receptor 4) activation in KLK1-mediated signaling in the development of

 DN. Yiu WH, Wong DW, Chan LY, Leung JC, Chan KW, Lan HY, Lai KN, Tang SC. Tissue Kallikrein Mediates Pro-Inflammatory 

Pathways and Activation of Protease-Activated Receptor-4 in Proximal Tubular Epithelial Cells. PLoS ONE (2014); 9(2): e88894

Renal Graft Research: In vitro renal epithelial cell culture models have provided a great deal of information about the outcome

 of renal clinical transplantation models. In a study carried out using Lonza’s Primary Human Renal Proximal Tubular Epithelial 

cells maintained in REGMTMTM renal epithelial growth media (cat# CC-2553 & CC-3190 respectively), the group study the

 effects of HCMV (Human cytomegalovirus) infection on renal cells. Masako Shimamura, Joanne E. Murphy-Ullrich and

 William J. Britt. Human Cytomegalovirus Induces TGF-β1 Activation in Renal Tubular Epithelial Cells after

 Epithelial-to-Mesenchymal Transition. PLoS Pathogens. (2010); 6(11): e1001170

 Storage and Content

1 x REBMTM Basal Medium (CC-3191), 500 mL

1 x REGMTM SinglequotsTM Supplement Pack (CC-4127) containing:

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Natural Cap Vial with Hydrocortisone, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

1 x Bottle FBS, 2.50 mL

1 x Natural Cap Vial with Transferrin, 0.50 mL

1 x Amber Vial with Triiodothyronine, 0.50 mL

1 x Amber Vial with Epinephrine, 0.50 mL

BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™

CC-3170

Culture system containing BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171) and BEGMTM Bronchial Epithelial

 Cell Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4175).

Co-culture of Asthmatic Bronchial Epithelial and Smooth Muscle Cells in Monolayer

Co-culture of asthmatic bronchial epithelial and smooth muscle cells grown in BEGM™ and SmGM™ 2 BulletKit™ and stained 

with anti-alpha-smooth muscle actin

Product Overview

BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial 

epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.

Published research shows BEGMTM BulletKitTM has expanded applications. Our customers have used BEGMTM BulletKitTM to

 culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models 

with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the 'Data' tab.

BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system 

that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to

primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within 

the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with 

normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since 

customers can use the same media to support multiple cell types.

Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells

El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to 

examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells

Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza's HUVEC and NHBE in a 

3D environment.

Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from 

non-transplanted normal lungs

Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit 

consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGMTM BulletKitTM Growth Media 

(catalog#. CC-3170).

Storage and Content

1 x BEBMTM Basal Medium (CC-3171), 500 mL

1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:

1 x Orange Cap Vial with BPE, 2.00 mL

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Natural Cap Vial with Hydrocortisone, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

1 x Amber Vial with Retinoic Acid, 0.50 mL

1 x Natural Cap Vial with Transferrin, 0.50 mL

1 x Amber Vial with Triiodothyronine, 0.50 mL

1 x Amber Vial with Epinephrine, 0.50 mL

1 x Green Cap Vial with hEGF, 0.50 mL

EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM

CC-3202

Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 MV Microvascular Endothelial Cell Growth Medium SingleQuotsTM

 supplements (CC-4147) required for growth of Microvascular Endothelial Cells

EGM® 2MV Microvascular Endothelial Cell Growth Medium-2 BulletKit®

Includes basal medium and SingleQuots® Kit

Product Overview

Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has

 its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used 

to grow CloneticsTM Primary Cells.    

The following Endothelial Cell Growth Medium BulletKitsTM are available:

EGMTM-2 BulletKitTM – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Final concentration of FBS in the mixed 

BulletKit is  2%.  Contains VEGF for rapid proliferation. Does not contain BBE.

EGMTM BulletKitTM – Optimized for HUVECs cat. no. CC-2517 and CC-2519. Contains BBE .Final concentration of FBS in the mixed BulletKit is 

2%. Does not contain exogenous VEGF.

EGMTM-Plus BulletKitTM – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains BBE. Final concentration 

of FBS in the mixed BulletKit is  2%. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.

EGMTM-2MV BulletKitTM – Coronary and Iliac artery, human microvascular endothelial cells. 

Final concentration of FBS in the mixed BulletKit is 5%.  Contains VEGF for rapid proliferation. Does not contain BBE.

Lonza guarantees the performance of CloneticsTM/PoieticsTM Cells only if appropriate CloneticsTM/PoieticsTM Media and Reagents are used 

exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including 

the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the 

appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

Storage and Content

1 x EBMTM-2 Basal Medium (CC-3156),  500 mL

1 x EGMTM-2 MV SingleQuotsTM Supplement Pack (CC-4147) containing:

1 x Bottle FBS, 25.00 mL

1 x Natural Cap Vial with Hydrocortisone, 0.20 mL

1 x Gray Cap Vial with hFGF-B, 2.00 mL

1 x White Cap Vial with VEGF, 0.50 mL

1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL

1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 m