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产品名称 GAD65 Antibody - ELISA (DLD-Diagnostika;CAT#EA104/96)
货号: EA104/96 规格: 12x8 Determinations
品牌: DLD-Diagnostika 价格: ¥请咨询
产品分类: ELISA试剂盒 研究领域: Internal medicine,immunology,endocrinology
CAS编号: N/A 分子量: N/A
分子式: N/A 别名: GAD65 Antibody - ELISA
储存条件: 4℃ 运输条件: 4℃
货期: 3-4周 应用: 科研
说明书: 请跟客服人员索取 备注:
产品描述
Product NameGAD65 Antibody - ELISA
Catalog-No.EA104/96
DescriptionEnzyme Immuno Assay for the Quantitative Determination of Antibodies against Glutamic Acid Decarboxylase in serum
Format12x8 Determinations, single break apart wells
ProcedureThe GAD65 Antibody ELISA depends on the ability of GAD autoantibodies to act divalently and form a bridge between GAD coated on ELISA plate wells and liquid phase GAD-biotin. The GAD-biotin bound is is then quantitated by addition of streptavidin peroxidase and a colorogenic substrate (TMB) with reading of final absorbance at 2 wavelengths (450 and 405 nm) to obtain maximum measuring range (0-2,000 units per ml of WHO reference preparation NIBSC 97/550).
Clinical FieldsInternal medicine, immunology, endocrinology
DiseasesInsulin Dependent Diabetes Mellitus (IDDM)
Normal Value99% of healthy blood donor sera give values of less than 5 Units per ml (NIBSC 97/550) in the assay suggesting that values of 5 U/ml or greater can be considered positive for GAD autoantibodies. However, a provisional cut off for positivity of 10 Units per ml is recommended until individual laboratories have established their own cut off values using appropriate control sera.
SpecificityIn the DASP 2005 study the kit achieved 98% (n=100) specificity.
SensitivityIn the DASP 2005 study the kit achieved 92% (n=50) sensitivity.


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