Zyppy Plasmid Miniprep Kit

Cat #          Name                                           Size

D4019        Zyppy Plasmid Miniprep Kit        100 Preps

D4020        Zyppy Plasmid Miniprep Kit        400 Preps

D4036        Zyppy Plasmid Miniprep Kit        50 Preps

D4037        Zyppy Plasmid Miniprep Kit        800 Preps

Brand ：Zymo Research

_r1011_zymoclean_gel_rna_recovery_kit.pdf

HIGHLIGHTS

Fastest: 8 minutes from culture flask to high-quality plasmid DNA.

Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.

High Quality: Ready for PCR, sequencing, cloning, and transfection.

DESCRIPTION

The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

TECHNICAL SRECIFICATIONS

Applicable For

Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.

Elution Volume

≥ 30 µl

Equipment

Microcentrifuge and/or a vacuum manifold.

Processing Time

8 min

Purity

Typical Abs260/280 ≥1.8.

Size Range

Up to 25 kb

Yield

Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.

Supplemental Info

How to Increase Plasmid Yield

DOs and DON'Ts of Plasmid Purification